SIRPA ELISA Kit
Quick Overview for SIRPA ELISA Kit (ABIN7830998)
Target
See all SIRPA ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
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Purpose
- SIRPA (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Sirp alpha1 expression in cultured cells.
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Analytical Method
- Qualitative
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Signal-Regulatory Protein alpha (SIRPA) ELISA Kit
SIRPA Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Signal-Regulatory Protein alpha (SIRPA) ELISA KitSIRPA Reactivity: Human Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Signal-Regulatory Protein alpha (SIRPA) ELISA KitSIRPA Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Lysate, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Uncoated
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Protocol
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- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store at 4°C for six months.
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- SIRPA (Signal-Regulatory Protein alpha (SIRPA))
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Alternative Name
- SIRPA
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Background
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Synonyms: BIT|CD172A|MFR|MYD-1|P84|PTPNS1|SHPS-1|SHPS1|SIRP|SIRP-ALPHA-1|SIRPalpha|SIRPalpha2
Gene Description: signal-regulatory protein alpha
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Gene ID
- 140885
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UniProt
- P78324
Target See all SIRPA ELISA Kits
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