SYT1 ELISA Kit

Catalog No. ABIN7831003

Human SYT1 ELISA Kit, Colorimetric assay for quantification of Human SYT1.

Quick Overview for SYT1 ELISA Kit (ABIN7831003)

Target: SYT1 (Synaptotagmin I (SYT1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details SYT1 ELISA Kit

Purpose: SYT1/SYT2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Synaptotagmin expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for SYT1

Target: SYT1 (Synaptotagmin I (SYT1))

Alternative Name: SYT1

Background:

Synonyms: DKFZp781D2042|P65|SVP65|SYT

Gene Description: synaptotagmin I

Gene ID: 6857

UniProt: P21579

Pathways: Synaptic Vesicle Exocytosis, Dicarboxylic Acid Transport