RRN3 ELISA Kit

Catalog No. ABIN7831005

The Human RRN3 ELISA Kit (ABIN7831005) is a Colorimetric ELISA Kit designed to quantify Human RRN3.

Quick Overview for RRN3 ELISA Kit (ABIN7831005)

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details RRN3 ELISA Kit

Purpose: RRN3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TIF-IA expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for RRN3

Target: RRN3 (RRN3 RNA Polymerase I Transcription Factor (RRN3))

Alternative Name: RRN3

Background:

Synonyms: DKFZp566E104|MGC104238|TIFIA

Gene Description: RRN3 RNA polymerase I transcription factor homolog (S. cerevisiae)

Gene ID: 54700

UniProt: Q9NYV6

Pathways: Negative Regulation of intrinsic apoptotic Signaling