TXNRD2 ELISA Kit

Catalog No. ABIN7831009

Human TXNRD2 ELISA Kit, Colorimetric assay for quantification of Human TXNRD2.

Quick Overview for TXNRD2 ELISA Kit (ABIN7831009)

Target: TXNRD2 (Thioredoxin Reductase 2 (TXNRD2))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details TXNRD2 ELISA Kit

Purpose: TXNRD2 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TRXR2 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for TXNRD2

Target: TXNRD2 (Thioredoxin Reductase 2 (TXNRD2))

Alternative Name: TXNRD2

Background:

Synonyms: SELZ|TR|TR-BETA|TR3|TRXR2

Gene Description: thioredoxin reductase 2

Gene ID: 10587

UniProt: Q9NNW7

Pathways: Cell RedoxHomeostasis