TUBA1A ELISA Kit

Catalog No. ABIN7831010

This Colorimetric ELISA kit is designed for the quantitative measurement of Human TUBA1A.

Quick Overview for TUBA1A ELISA Kit (ABIN7831010)

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details TUBA1A ELISA Kit

Purpose: TUBA1A (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of Tubulin alpha expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for TUBA1A

Target: TUBA1A (Tuba1a) (Tubulin, alpha 1a (Tuba1a))

Alternative Name: TUBA1A

Background:

Synonyms: B-ALPHA-1|FLJ25113|LIS3|TUBA3

Gene Description: tubulin, alpha 1a

Gene ID: 7846

UniProt: Q71U36

Pathways: Microtubule Dynamics, M Phase