VAV3 ELISA Kit

Catalog No. ABIN7831011

The Human VAV3 ELISA Kit (ABIN7831011) is a Colorimetric ELISA Kit designed to quantify Human VAV3.

Quick Overview for VAV3 ELISA Kit (ABIN7831011)

Target: VAV3 (Vav 3 Oncogene (VAV3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Product Details VAV3 ELISA Kit

Purpose: VAV3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of VAV3 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for VAV3

Target: VAV3 (Vav 3 Oncogene (VAV3))

Alternative Name: VAV3

Background:

Synonyms: FLJ40431

Gene Description: vav 3 guanine nucleotide exchange factor

Gene ID: 10451

Pathways: Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway