Ezrin ELISA Kit
Quick Overview for Ezrin ELISA Kit (ABIN7831014)
Target
See all Ezrin (EZR) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
-
-
Purpose
- EZR (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of EZR expression in cultured cells.
-
Analytical Method
- Qualitative
-
-
Ezrin (EZR) ELISA Kit
EZR Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Ezrin (EZR) ELISA KitEZR Reactivity: Mouse Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Ezrin (EZR) ELISA KitEZR Reactivity: Rat Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Ezrin (EZR) ELISA KitEZR Reactivity: Human Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum
Ezrin (EZR) ELISA KitEZR Reactivity: Human Colorimetric Sandwich ELISA 0.31 ng/mL - 20 ng/mL Cell Lysate, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Uncoated
-
Protocol
-
- Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
- Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
- HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
- TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.
-
Restrictions
- For Research Use only
-
-
-
Storage
- 4 °C
-
Storage Comment
- Store the kit at 4°C.
-
-
- Ezrin (EZR)
-
Alternative Name
- EZR
-
Background
-
Synonyms: CVIL|CVL|DKFZp762H157|FLJ26216|MGC1584|VIL2
Gene Description: ezrin
-
Gene ID
- 7430
-
Pathways
- Maintenance of Protein Location
Target See all Ezrin (EZR) ELISA Kits
-
