DDIT3 ELISA Kit

Catalog No. ABIN7831015

Human DDIT3 ELISA Kit, Colorimetric assay for quantification of Human DDIT3.

Quick Overview for DDIT3 ELISA Kit (ABIN7831015)

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Adherent Cell Culture, Cell Culture Cells, Cell Samples

Product Details DDIT3 ELISA Kit

Purpose: DDIT3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of DDIT3 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store the kit at 4°C.

Target Details for DDIT3

Target: DDIT3 (DNA-Damage-Inducible Transcript 3 (DDIT3))

Alternative Name: DDIT3

Background:

Synonyms: CEBPZ|CHOP|CHOP10|GADD153|MGC4154

Gene Description: DNA-damage-inducible transcript 3

Gene ID: 1649

Pathways: Regulation of Muscle Cell Differentiation, ER-Nucleus Signaling, Skeletal Muscle Fiber Development, Cell RedoxHomeostasis