TP53I11 ELISA Kit

Catalog No. ABIN7831024

Human TP53I11 ELISA Kit, Colorimetric assay for quantification of Human TP53I11.

Quick Overview for TP53I11 ELISA Kit (ABIN7831024)

Target: TP53I11 (Tumor Protein P53 Inducible Protein 11 (TP53I11))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details TP53I11 ELISA Kit

Purpose: TP53I11 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of TP53I11 expression in cultured cells.

Analytical Method: Qualitative

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for TP53I11

Target: TP53I11 (Tumor Protein P53 Inducible Protein 11 (TP53I11))

Alternative Name: TP53I11

Background:

Synonyms: PIG11

Gene Description: tumor protein p53 inducible protein 11

Gene ID: 9537

UniProt: O14683