NDC80 ELISA Kit

Catalog No. ABIN7831026

The Human NDC80 ELISA Kit (ABIN7831026) is a Colorimetric ELISA Kit designed to quantify Human NDC80.

Quick Overview for NDC80 ELISA Kit (ABIN7831026)

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details NDC80 ELISA Kit

Purpose: NDC80 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of NDC80 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for NDC80

Target: NDC80 (NDC80 Kinetochore Complex Component Homolog (NDC80))

Alternative Name: NDC80

Background:

Synonyms: HEC|HEC1|KNTC2|TID3|hsNDC80

Gene Description: NDC80 homolog, kinetochore complex component (S. cerevisiae)

Gene ID: 10403

UniProt: O14777

Pathways: Maintenance of Protein Location