CLOCK ELISA Kit

Catalog No. ABIN7831035

The Human CLOCK ELISA Kit (ABIN7831035) is a Colorimetric ELISA Kit designed to quantify Human CLOCK.

Quick Overview for CLOCK ELISA Kit (ABIN7831035)

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details CLOCK ELISA Kit

Purpose: CLOCK (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of CLOCK expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for CLOCK

Target: CLOCK (Clock Homolog (Mouse) (CLOCK))

Alternative Name: CLOCK

Background:

Synonyms: KAT13D|KIAA0334|bHLHe8

Gene Description: clock homolog (mouse)

Gene ID: 9575

UniProt: O15516

Pathways: Regulation of Lipid Metabolism by PPARalpha, Photoperiodism