MAP3K13 ELISA Kit

Catalog No. ABIN7831036

Human MAP3K13 ELISA Kit, Colorimetric assay for quantification of Human MAP3K13.

Quick Overview for MAP3K13 ELISA Kit (ABIN7831036)

Target: MAP3K13 (Mitogen-Activated Protein Kinase Kinase Kinase 13 (MAP3K13))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details MAP3K13 ELISA Kit

Purpose: MAP3K13 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of MAP3K13 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for MAP3K13

Target: MAP3K13 (Mitogen-Activated Protein Kinase Kinase Kinase 13 (MAP3K13))

Alternative Name: MAP3K13

Background:

Synonyms: LZK|MGC133196

Gene Description: mitogen-activated protein kinase kinase kinase 13

Gene ID: 9175

UniProt: O43283

Pathways: Signaling Events mediated by VEGFR1 and VEGFR2