AKAP10 ELISA Kit

Catalog No. ABIN7831039

Human AKAP10 ELISA Kit, Colorimetric assay for quantification of Human AKAP10.

Quick Overview for AKAP10 ELISA Kit (ABIN7831039)

Target: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details AKAP10 ELISA Kit

Purpose: AKAP10 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of AKAP10 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse, Rat

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for AKAP10

Target: AKAP10 (A Kinase (PRKA) Anchor Protein 10 (AKAP10))

Alternative Name: AKAP10

Background:

Synonyms: D-AKAP2|MGC9414|PRKA10

Gene Description: A kinase (PRKA) anchor protein 10

Gene ID: 11216

UniProt: O43572

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling