ZIC1 ELISA Kit

Catalog No. ABIN7831044

The Human ZIC1 ELISA Kit (ABIN7831044) is a Colorimetric ELISA Kit designed to quantify Human ZIC1.

Quick Overview for ZIC1 ELISA Kit (ABIN7831044)

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details ZIC1 ELISA Kit

Purpose: ZIC1/ZIC2/ZIC3 (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of ZIC1/ZIC2/ZIC3 expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for ZIC1

Target: ZIC1 (Zinc Finger Protein ZIC 1 (ZIC1))

Alternative Name: ZIC1

Background:

Synonyms: ZIC|ZNF201

Gene Description: Zic family member 1 (odd-paired homolog, Drosophila)

Gene ID: 7545

UniProt: Q15915