LMX1B ELISA Kit

Catalog No. ABIN7831047

The Human LMX1B ELISA Kit (ABIN7831047) is a Colorimetric ELISA Kit designed to quantify Human LMX1B.

Quick Overview for LMX1B ELISA Kit (ABIN7831047)

Target: LMX1B (LIM Homeobox Transcription Factor 1, beta (LMX1B))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Cell ELISA

Application: ELISA

Sample Type: Cell Samples, Suspension Cell Culture

Product Details LMX1B ELISA Kit

Purpose: LMX1B (Human) Cell-Based ELISA Kit is an indirect enzyme-linked immunoassay for qualitative determination of LMX1B expression in cultured cells.

Analytical Method: Qualitative

Cross-Reactivity: Mouse

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Uncoated

Protocol:

1. Cells are seeded onto the bottom of each well. The cells are quenched, fixed and the well is blocked.
2. Primary antibodies specific for the target antigen are added and allowed to bind to their respective epitopes.
3. HRP-conjugated secondary antibodies specific for the primary antibody are added and allowed to bind to their respective epitopes.
4. TMB substrate is converted to the blue TMB diimine via the HRP enzyme. Upon addition of acid, the reaction terminates and the wells can be read at 450 nm.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 4°C for six months.

Target Details for LMX1B

Target: LMX1B (LIM Homeobox Transcription Factor 1, beta (LMX1B))

Alternative Name: LMX1B

Background:

Synonyms: LMX1.2|MGC138325|MGC142051|NPS1

Gene Description: LIM homeobox transcription factor 1, beta

Gene ID: 4010

UniProt: O60663

Pathways: Dopaminergic Neurogenesis