D-Dimer ELISA Kit
Quick Overview for D-Dimer ELISA Kit (ABIN7832835)
Target
See all D-Dimer ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA D2D (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human D2D (D-Dimer) in plasma.
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Analytical Method
- Quantitative
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D-Dimer ELISA Kit
Reactivity: Mouse Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma
D-Dimer ELISA KitReactivity: Human Colorimetric Competition ELISA 617.3 pg/mL - 50000 pg/mL Plasma
D-Dimer ELISA KitReactivity: Human Colorimetric Sandwich ELISA 78.13 ng/mL - 5000 ng/mL Plasma
D-Dimer ELISA KitReactivity: Rat Colorimetric Competition ELISA 123.5 pg/mL - 10000 pg/mL Plasma
D-Dimer ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
D-Dimer ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
D-Dimer ELISA KitReactivity: Human Colorimetric Sandwich ELISA 15.6 ng/mL - 1000 ng/mL Plasma, Serum
D-Dimer ELISA KitWide Range Reactivity: Mouse Colorimetric Competition ELISA 61.7 ng/mL - 5000 ng/mL Plasma
D-Dimer ELISA KitReactivity: Rat Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
D-Dimer ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 7.813 ng/mL - 500 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C
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Storage Comment
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- D-Dimer
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Alternative Name
- D2D
Target See all D-Dimer ELISA Kits
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