IgG3 ELISA Kit
Quick Overview for IgG3 ELISA Kit (ABIN7832844)
Target
See all IgG3 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA IgG3 (Mouse) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Mouse IgG3 (Immunoglobulin G3) in serum, plasma.
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Analytical Method
- Quantitative
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IgG3 ELISA Kit
Reactivity: Human Colorimetric Sandwich ELISA 0.156 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
IgG3 ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 12.5 ng/mL - 400 ng/mL Plasma, Serum, Urine
IgG3 ELISA KitReactivity: Mouse Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
IgG3 ELISA KitReactivity: Human Colorimetric Competition ELISA 2.7-2000 ng/mL Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C
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Storage Comment
- Store at 2-8C for 6 months.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- IgG3
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Target Type
- Antibody
Target See all IgG3 ELISA Kits
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