NPPB ELISA Kit

Catalog No. ABIN7832853

The Human NPPB ELISA Kit (ABIN7832853) is a Colorimetric ELISA Kit designed to quantify Human NPPB.

Quick Overview for NPPB ELISA Kit (ABIN7832853)

Target: NPPB (Natriuretic Peptides B (NPPB))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Sample Type: Plasma, Serum

Grade: QuickTest

Product Details NPPB ELISA Kit

Purpose: FastELISA NPPB (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human NPPB (Brain natriuretic peptide, BNP) in Serum, plasma.

Analytical Method: Quantitative

Specificity: This assay is highly specific for detection of Human NPPB. No significant cross-reactivity or interference between Human NPPB and analogues was observed.

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Plate: Pre-coated

Protocol:

1. After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
2. Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
3. Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.

Restrictions: For Research Use only

Handling

Handling Advice: Avoid repeated freezing and thawing

Storage: 4 °C,-20 °C

Storage Comment: Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.

Expiry Date: 6 months

Target Details for NPPB

Target: NPPB (Natriuretic Peptides B (NPPB))

Alternative Name: NPPB

Background:

Synonyms: BNP

Gene Description: natriuretic peptide precursor B

Gene ID: 4879

Pathways: Hormone Activity