TNNI3 ELISA Kit
Quick Overview for TNNI3 ELISA Kit (ABIN7832857)
Target
See all TNNI3 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
-
-
Purpose
- FastELISA TNNI3 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human TNNI3 (Cardiac troponin I) in Serum, plasma.
-
Analytical Method
- Quantitative
-
Specificity
- This assay is highly specific for detection of Human TNNI3. No significant cross-reactivity or interference between Human TNNI3 and analogues was observed.
-
-
Cardiac Troponin I (TNNI3) ELISA Kit
TNNI3 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Rat Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Mouse Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Mouse Colorimetric Sandwich ELISA 7.813 pg/mL - 500 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitHigh Sensitivity TNNI3 Reactivity: Human Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Dog Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Cow Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Chicken Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Cardiac Troponin I (TNNI3) ELISA KitTNNI3 Reactivity: Guinea Pig Colorimetric Sandwich ELISA 15.625 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
-
-
Application Notes
- Optimal working dilution should be determined by the investigator.
-
Plate
- Pre-coated
-
Protocol
-
- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
-
Restrictions
- For Research Use only
-
-
-
Handling Advice
- Avoid repeated freezing and thawing
-
Storage
- 4 °C,-20 °C
-
Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
-
Expiry Date
- 6 months
-
-
- TNNI3 (Cardiac Troponin I (TNNI3))
-
Alternative Name
- TNNI3
-
Background
-
Synonyms: CMD2A|CMH7|MGC116817|RCM1|TNNC1|cTnI
Gene Description: troponin I type 3 (cardiac)
-
Gene ID
- 7137
Target See all TNNI3 ELISA Kits
-
