Azurocidin ELISA Kit
Quick Overview for Azurocidin ELISA Kit (ABIN7832863)
Target
See all Azurocidin (AZU1) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA AZU1 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human AZU1 (Azurocidin, Heparin binding protein) in Serum.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human AZU1. No significant cross-reactivity or interference between Human AZU1 and analogues was observed.
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Azurocidin 1 (AZU1) ELISA Kit
AZU1 Reactivity: Human Colorimetric Sandwich ELISA 7.8 pg/mL - 500 pg/mL Cell Culture Supernatant, Serum, Tissue Homogenate, Urine
Azurocidin 1 (AZU1) ELISA KitAZU1 Reactivity: Human Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Azurocidin 1 (AZU1) ELISA KitAZU1 Reactivity: Human AA 27-248 Colorimetric Sandwich ELISA 62.5-4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Azurocidin 1 (AZU1) ELISA KitAZU1 Reactivity: Human Colorimetric Sandwich ELISA 156 pg/mL - 10000 pg/mL Cell Culture Supernatant, Plasma, Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- Azurocidin (AZU1) (Azurocidin 1 (AZU1))
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Alternative Name
- AZU1
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Background
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Synonyms: AZAMP|AZU|CAP37|HBP|HUMAZUR|NAZC
Gene Description: azurocidin 1
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Gene ID
- 566
Target See all Azurocidin (AZU1) ELISA Kits
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