CXCL9 ELISA Kit
Quick Overview for CXCL9 ELISA Kit (ABIN7832868)
Target
See all CXCL9 ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA CXCL9 (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human CXCL9 (Monokine induced by interferon gamma, MIG) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human CXCL9. No significant cross-reactivity or interference between Human CXCL9 and analogues was observed.
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gamma-Interferon-Induced Monokine (CXCL9) ELISA Kit
CXCL9 Reactivity: Rat Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Human Colorimetric Sandwich ELISA 16-1000 pg/mL Cell Lysate, Plasma, Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Human AA 23-125 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Mouse AA 22-126 Colorimetric Sandwich ELISA 31.2-2000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Pig Colorimetric Sandwich ELISA 5-1.400 pg/mL Cell Culture Supernatant, Plasma, Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Human Colorimetric Sandwich ELISA 31.2 pg/mL - 2000 pg/mL Cell Culture Supernatant, Plasma (EDTA - heparin), Serum
gamma-Interferon-Induced Monokine (CXCL9) ELISA KitCXCL9 Reactivity: Mouse Colorimetric Sandwich ELISA 62.5 pg/mL - 4000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Serum
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- CXCL9 (gamma-Interferon-Induced Monokine (CXCL9))
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Alternative Name
- CXCL9
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Background
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Synonyms: CMK|Humig|MIG|SCYB9|crg-10
Gene Description: chemokine (C-X-C motif) ligand 9
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Gene ID
- 4283
Target See all CXCL9 ELISA Kits
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