Myeloperoxidase ELISA Kit
Quick Overview for Myeloperoxidase ELISA Kit (ABIN7832870)
Target
See all Myeloperoxidase (MPO) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA MPO (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human MPO (Myeloperoxidase) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human MPO. No significant cross-reactivity or interference between Human MPO and analogues was observed.
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Myeloperoxidase (MPO) ELISA Kit
MPO Reactivity: Mouse Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Rat Colorimetric Sandwich ELISA 3.12 ng/mL - 200 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Mouse Colorimetric Sandwich ELISA 31.25 pg/mL - 2000 pg/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Human Colorimetric Sandwich ELISA 7.8 ng/mL - 500 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Cow Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Pig Colorimetric Sandwich ELISA 78 pg/mL - 5000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Rat Colorimetric Sandwich ELISA 0.781 ng/mL - 50 ng/mL Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Horse Colorimetric Sandwich ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Dog Colorimetric Sandwich ELISA 31.25 ng/mL - 2000 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Myeloperoxidase (MPO) ELISA KitMPO Reactivity: Human AA 49-745 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Cell Lysate, Plasma (EDTA), Plasma (heparin), Saliva, Serum, Tissue Homogenate, Urine
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- Myeloperoxidase (MPO)
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Alternative Name
- MPO
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Background
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Synonyms: -
Gene Description: myeloperoxidase
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Gene ID
- 4353
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Pathways
- Chromatin Binding
Target See all Myeloperoxidase (MPO) ELISA Kits
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