Prostate Specific Antigen ELISA Kit
Quick Overview for Prostate Specific Antigen ELISA Kit (ABIN7832879)
Target
See all Prostate Specific Antigen (PSA) ELISA KitsReactivity
Detection Method
Method Type
Application
Sample Type
Grade
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Purpose
- FastELISA PSA (Human) ELISA Kit is a sandwich enzyme immunoassay for the quantitative measurement of Human PSA (ProstateSpecific Antigen) in Serum, plasma.
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Analytical Method
- Quantitative
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Specificity
- This assay is highly specific for detection of Human PSA. No significant cross-reactivity or interference between Human PSA and analogues was observed.
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Prostate Specific Antigen (PSA) ELISA Kit
PSA Reactivity: Rat Colorimetric Sandwich ELISA 0.15 ng/mL - 10 ng/mL Plasma, Serum, Tissue Homogenate
Prostate Specific Antigen (PSA) ELISA KitPSA Reactivity: Human Colorimetric Sandwich ELISA 0.2 ng/mL - 12.5 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Prostate Specific Antigen (PSA) ELISA KitPSA Reactivity: Mouse Colorimetric Sandwich ELISA 0.16 ng/mL - 10 ng/mL Cell Culture Supernatant, Plasma, Serum
Prostate Specific Antigen (PSA) ELISA KitPSA Reactivity: Mouse Colorimetric Sandwich ELISA 15.6 pg/mL - 1000 pg/mL Plasma, Serum, Tissue Homogenate
Prostate Specific Antigen (PSA) ELISA KitPSA Reactivity: Mouse Colorimetric Sandwich ELISA 0.312 ng/mL - 20 ng/mL Plasma, Serum, Tissue Homogenate
Prostate Specific Antigen (PSA) ELISA KitPSA Reactivity: Human Colorimetric Sandwich ELISA 938-60000 pg/mL Cell Lysate, Plasma, Serum
Prostate Specific Antigen (PSA) ELISA KitPSA Reactivity: Human AA 18-261 Colorimetric Sandwich ELISA 312-20000 pg/mL Cell Culture Supernatant, Plasma (EDTA), Plasma (heparin), Serum
Prostate Specific Antigen (PSA) ELISA KitPSA Reactivity: Mouse Colorimetric Sandwich ELISA 0.312-20 ng/mL Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal working dilution should be determined by the investigator.
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Plate
- Pre-coated
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Protocol
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- After the kit is equilibrated at room temperature, add 50 μL Standard/Sample Diluent Buffer to each Standard well, and add 50 μL sample to the sample well. Immediately add 50 μL 1X HRP Conjugate Antibody Solution to each well. Incubate at 37 °C on microplate oscillator for 60 min.
- Discard the liquid in the plate, add 200 μL 1x Wash Buffer to each well, and wash the plate 5 times. After pat it dry against clean absorbent paper, add 90 μL TMB Substrate Solution to each well, incubate at 37 °C for 20 minutes in the dark
- Add 50 μL Stop Solution to each well, shake plate on a plate shaker for 1 minute to mix. Record the OD at 450 nm immediately, calculation of the results.
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Restrictions
- For Research Use only
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Handling Advice
- Avoid repeated freezing and thawing
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Storage
- 4 °C,-20 °C
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Storage Comment
- Store at 2-8C for 6 months. For long term storage store at -20°C.Avoid repeated freezing and thawing.
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Expiry Date
- 6 months
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- Prostate Specific Antigen (PSA)
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Alternative Name
- PSA
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Pathways
- Complement System
Target See all Prostate Specific Antigen (PSA) ELISA Kits
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