alpha Fetoprotein ELISA Kit
Quick Overview for alpha Fetoprotein ELISA Kit (ABIN7832945)
Target
See all alpha Fetoprotein (AFP) ELISA KitsReactivity
Detection Method
Method Type
Detection Range
Application
Sample Type
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Minimum Detection Limit
- 781 pg/mL
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Purpose
- Sandwich ELISA for quantitative detection of Human Alpha-fetoglobulin concentrations.
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Analytical Method
- Quantitative
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Sensitivity
- 280 pg/mL
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Components
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- Coated 96-well Plate
- Standard
- Biotin-Labeled Detection Antibody (100X)
- Streptavidin-HRP (100X)
- Standard/Sample Diluent
- Detection Antibody Diluent
- Streptavidin-HRP Diluent
- Wash Buffer (20X)
- TMB Substrate Solution
- Stop Solution
- Plate Adhesive Strips
- Technical Manual
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Material not included
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- Microplate reader capable of measuring absorbance at 450 nm.
- Adjustable pipettes and pipette tips to deliver 2 µl to 1 ml volumes.
- Adjustable 1-25 ml pipettes for reagent preparation.
- 100 ml and 1 liter graduated cylinders.
- Absorbent paper.
- Distilled or deionized water.
- Computer and software for ELISA data analysis.
- Tubes to prepare standard or sample dilutions.
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alpha-Fetoprotein (AFP) ELISA Kit
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Sample Volume
- 100 μL
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Plate
- Pre-coated
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Protocol
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- Prepare all reagents, samples and standards
- Add 100 µL Standard or Sample
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Biotin-Labeled Detection Antibody Working Solution
- Wash plate 3 times with Wash Buffer Working Solution
- Add 100 µL Steptavidin-HRP Working Solution
- Wash plate 5 times with Wash Buffer Working Solution
- Add 100 µL TMB Substrat Solution
- Add 100 µL Stop Solution
- Read the plate at 450 nm
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Restrictions
- For Research Use only
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Handling Advice
- Avoid freeze/thaw cycles
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Storage
- 4 °C,-20 °C
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Storage Comment
- Shipped and store at 4°C for 6 months, store at -20°C for one year. Avoid freeze/thaw cycles.
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Expiry Date
- 12 months
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- alpha Fetoprotein (AFP) (alpha-Fetoprotein (AFP))
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Alternative Name
- AFP
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Background
- Alternative Names: HPAFP, Alpha-fetoprotein, Alpha-1-fetoprotein, Alpha-fetoglobulin
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Gene ID
- 174
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UniProt
- P02771
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Pathways
- C21-Steroid Hormone Metabolic Process
Target See all alpha Fetoprotein (AFP) ELISA Kits
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