Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit
Quick Overview for Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit (ABIN7849987)
Target
Detection Method
Application
Sample Type
Analytical Method
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Detection Range
- 0.14 mmol/L - 4 mmol/L
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Minimum Detection Limit
- 0.14 mmol/L
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Purpose
- Free Fatty Acids (NEFA/FFA) Colorimetric Assay Kit
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Characteristics
- Free fatty acids (NEFA/FFA) generate acyl-CoA under the action of acyl synthase, then acyl-CoA generates hydrogen peroxide under the oxidation of acyl oxidase. Hydrogen peroxide reacts in the presence of enzymes and chromogens, producing a red substance with maximum absorption at 546 nm. The amount of this red product is proportional to the content of free fatty acids.
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Assay Time
- 0.5 h
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Assay Precision
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Inter-assay precision: 2.5-6.1%
Intra-assay precision: 1.5-4.5%
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Restrictions
- For Research Use only
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Handling Advice
- Protect from light.
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Storage
- 4 °C,-20 °C
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Storage Comment
- This product can be stored at 2~8°C/-20°C for 12 months with shading light. Please refer to the manual for the specific storage condition of the components.
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Expiry Date
- 12 months
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- Free Fatty Acid (FFA)
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Alternative Name
- Free Fatty Acids
Target
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