Pyruvate Kinase (PK) Activity Assay Kit

Catalog No. ABIN7850078

Quick Overview for Pyruvate Kinase (PK) Activity Assay Kit (ABIN7850078)

Target: PK

Detection Method: Colorimetric

Application: Biochemical Assay (BCA)

Sample Type: Cell Samples, Plasma, Serum, Tissue Samples

Analytical Method: Quantitative

Product Details

Detection Range: 0.08 U/L - 6.79 U/L

Minimum Detection Limit: 0.08 U/L

Purpose: Pyruvate Kinase (PK) Activity Assay Kit

Characteristics: In the presence of adenosine diphosphate (ADP), pyruvate kinase (PK) catalyzes the conversion of phosphoenolpyruvate (PEP) to pyruvate, Lactate dehydrogenase catalyzes the reaction of pyruvic acid with NADH to produce lactic acid and NAD+. The activity of pyruvate kinase can be calculated by detection the absorbance changes at 340 nm.

Application Details

Assay Time: 0.5 h

Assay Precision:

Inter-assay precision: 7%

Intra-assay precision: 5%

Restrictions: For Research Use only

Handling

Handling Advice: Protect from light.

Storage: -20 °C

Storage Comment: This product can be stored at -20°C for 12 months with shading light.

Expiry Date: 12 months

Target Details

Target: PK