EPO ELISA Kit

Catalog No. ABIN921054

Product Details EPO ELISA Kit

Target: EPO (Erythropoietin (EPO))

Binding Specificity: AA 27-192

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 46.9-3000 pg/mL

Minimum Detection Limit: 46.9 pg/mL

Application: ELISA

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse EPO

Brand: PicoKine™

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Tissue Homogenate

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A27-R192

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <15pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A27-R192

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Tissue Specificity: Produced by kidney or liver of adult mammals and by liver of fetal or neonatal mammals.

Plate: Pre-coated

Protocol: mouse EPO ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for EPO has been precoated onto 96-well plates. Standards(NSO, A27-R192)and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for EPO is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse EPO amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 3000pg/mL, 1500pg/mL, 750pg/mL,375.pg/mL, 187.5pg/mL, 93.8pg/mL, 46.9pg/mL mouse EPO standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernatants, serum, plasma(heparin) or tissue lysates to each empty well. See "Sample Dilution Guideline" above for details. We recommend that each mouse EPO standard solution and each sample is measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 225, Standard deviation: 10.13, CV(%): 4.5
• Sample 2: n=16, Mean(pg/ml): 457, Standard deviation: 17.4, CV(%): 3.8
• Sample 3: n=16, Mean(pg/ml): 1046, Standard deviation: 55.4, CV(%): 5.3,
• Sample 1: n=24, Mean(pg/ml): 246, Standard deviation: 23.12, CV(%): 9.4
• Sample 2: n=24, Mean(pg/ml): 486, Standard deviation: 36.45, CV(%): 7.5
• Sample 3: n=24, Mean(pg/ml): 1118, Standard deviation: 43.6, CV(%): 3.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for EPO

Target: EPO (Erythropoietin (EPO))

Alternative Name: EPO (EPO Products)

Target Type: Hormone

Background:

Protein Function: Erythropoietin is the principal hormone involved in the regulation of erythrocyte differentiation and the maintenance of a physiological level of circulating erythrocyte mass.

Background: Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte(red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.

Synonyms: Erythropoietin,Epo,

Full Gene Name: Erythropoietin

Cellular Localisation: Secreted.

Gene ID: 13856

UniProt: P07321

Pathways: JAK-STAT Signaling, Hormone Activity, Negative Regulation of intrinsic apoptotic Signaling, Negative Regulation of Transporter Activity