MMP3 ELISA Kit

Catalog No. ABIN921069

This Mouse MMP3 ELISA Kit is a Colorimetric ELISA Kit designed to quantify Mouse MMP3 in Cell Culture Supernatant, Serum and Plasma (heparin). This ELISA Kit has been cited in 3+ publications.

Quick Overview for MMP3 ELISA Kit (ABIN921069)

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Binding Specificity: AA 18-477

Reactivity: Mouse

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 156-10000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin)

Product Details MMP3 ELISA Kit

Minimum Detection Limit: 156 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse MMP-3

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: Y18-C477

Cross-Reactivity (Details): There is cross-reactivity with MMP-10 approximately 2 % and no detectable cross-reactivity with other MMPs.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: Y18-C477

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the peptidase M10A family.

Plate: Pre-coated

Protocol: mouse MMP-3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for MMP-3 has been precoated onto 96-well plates. Standards(NSO, Y18-C477) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for MMP-3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse MMP-3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL, 156pg/mL mouse MMP-3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of mouse cell culture supernates, serum or plasma( heparin) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each mouse MMP-3 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(pg/ml): 1385, Standard deviation: 76.2, CV(%): 5.5
• Sample 2: n=16, Mean(pg/ml): 3758, Standard deviation: 233, CV(%): 6.2
• Sample 3: n=16, Mean(pg/ml): 6772, Standard deviation: 291.2, CV(%): 4.3,
• Sample 1: n=24, Mean(pg/ml): 1479, Standard deviation: 99.1, CV(%): 6.7
• Sample 2: n=24, Mean(pg/ml): 3966, Standard deviation: 293.5, CV(%): 7.4
• Sample 3: n=24, Mean(pg/ml): 7247, Standard deviation: 427.6, CV(%): 5.9

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

References for MMP3 ELISA Kit (ABIN921069)

Xie, Huo, Li, Dai, Xu, Yin: "Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the Müller Cell Notch Signaling Pathway." in: Cell transplantation, Vol. 26, Issue 6, pp. 967-982, (2018) (PubMed).

Ågren, Schnabel, Christensen, Mirastschijski: "Tumor necrosis factor-?-accelerated degradation of type I collagen in human skin is associated with elevated matrix metalloproteinase (MMP)-1 and MMP-3 ex vivo." in: European journal of cell biology, Vol. 94, Issue 1, pp. 12-21, (2014) (PubMed).

Zhao, Fan, Zhang, Sun, Li, Xiong, Zhang, Fan: "Chitosan-plasmid DNA nanoparticles encoding small hairpin RNA targeting MMP-3 and -13 to inhibit the expression of dedifferentiation related genes in expanded chondrocytes." in: Journal of biomedical materials research. Part A, Vol. 102, Issue 2, pp. 373-80, (2013) (PubMed).

Target Details for MMP3

Target: MMP3 (Matrix Metallopeptidase 3 (Stromelysin 1, Progelatinase) (MMP3))

Alternative Name: MMP3

Background:

Protein Function: Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V, collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase.

Background: Matrix metalloproteinase-3(MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase(MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54 % identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.

Synonyms: Stromelysin-1,SL-1,3.4.24.17,EMS-2,Matrix metalloproteinase-3,MMP-3,Transin-1,Mmp3,

Full Gene Name: Stromelysin-1

Cellular Localisation: Secreted, extracellular space, extracellular matrix.

Gene ID: 17392

UniProt: P28862