Prostate Specific Antigen ELISA Kit

Catalog No. ABIN921102

This Human Prostate Specific Antigen ELISA Kit is a Colorimetric ELISA Kit designed to quantify Human Prostate Specific Antigen.

Quick Overview for Prostate Specific Antigen ELISA Kit (ABIN921102)

Target: Prostate Specific Antigen (PSA)

Binding Specificity: AA 18-261

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Detection Range: 312-20000 pg/mL

Application: ELISA

Sample Type: Cell Culture Supernatant, Serum, Plasma (heparin), Plasma (EDTA)

Product Details Prostate Specific Antigen ELISA Kit

Minimum Detection Limit: 312 pg/mL

Purpose: Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human Kallikrein 3

Brand: PicoKine™

Analytical Method: Quantitative

Specificity: Expression system for standard: NSO
Immunogen sequence: A18-P261

Cross-Reactivity (Details): There is no detectable cross-reactivity with other relevant proteins.

Sensitivity: <10pg/mL

Material not included: Microplate reader in standard size. Automated plate washer. Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection. Clean tubes and Eppendorf tubes. Washing buffer (neutral PBS or TBS). Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g Nacl

Immunogen: Expression system for standard: NSO
Immunogen sequence: A18-P261

Application Details

Application Notes: Before using Kit, spin tubes and bring down all components to bottom of tube. Duplicate well assay was recommended for both standard and sample testing.

Comment:

Sequence similarities: Belongs to the peptidase S1 family. Kallikrein subfamily.

Plate: Pre-coated

Protocol: human Kallikrein 3 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from mouse specific for Kallikrein 3 has been precoated onto 96-well plates. Standards(NSO, A18-P261) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for Kallikrein 3 is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human Kallikrein 3 amount of sample captured in plate.

Assay Procedure:

Aliquot 0.1 mL per well of the 20000pg/mL, 10000pg/mL, 5000pg/mL, 2500pg/mL, 1250pg/mL, 625pg/mL, 312pg/mL human Kallikrein 3 standard solutions into the precoated 96-well plate. Add 0.1 mL of the sample diluent buffer into the control well (Zero well). Add 0.1 mL of each properly diluted sample of human cell culture supernates, serum or plasma(heparin, EDTA) to each empty well. See "Sample Dilution Guideline" above for details. It is recommended that each human Kallikrein 3 standard solution and each sample be measured in duplicate.

Assay Precision:

• Sample 1: n=16, Mean(ng/ml): 1.8, Standard deviation: 0.108, CV(%): 6
• Sample 2: n=16, Mean(ng/ml): 6.7, Standard deviation: 0.422, CV(%): 6.3
• Sample 3: n=16, Mean(ng/ml): 12.7, Standard deviation: 0.686, CV(%): 5.4,
• Sample 1: n=24, Mean(ng/ml): 2.6, Standard deviation: 0.203, CV(%): 7.8
• Sample 2: n=24, Mean(ng/ml): 8.4, Standard deviation: 0.58, CV(%): 6.9
• Sample 3: n=24, Mean(ng/ml): 14.7, Standard deviation: 0.853, CV(%): 5.8

Restrictions: For Research Use only

Handling

Handling Advice: Avoid multiple freeze-thaw cycles.

Storage: -20 °C,4 °C

Storage Comment: Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles

Expiry Date: 12 months

Target Details for Prostate Specific Antigen

Target: Prostate Specific Antigen (PSA)

Alternative Name: KLK3

Background:

Protein Function: Hydrolyzes semenogelin-1 thus leading to the liquefaction of the seminal coagulum.

Background: Prostate-specific antigen(PSA), also known as kallikrein III, seminin, semenogelase, gamma-seminoprotein and P-30 antigen) is a 34 kD glycoprotein manufactured almost exclusively by the prostate gland, PSA is produced for the ejaculate where it liquifies the semen in the seminal coagulum and allows sperm to swim freely.1It is also believed to be instrumental in dissolving the cervical mucous cap, allowing the entry of sperm.2It is a serine protease enzyme, the gene of which is located on the nineteenth chromosome(19q13).3

Synonyms: Prostate-specific antigen,PSA,3.4.21.77,Gamma-seminoprotein,Seminin,Kallikrein-3,P-30 antigen,Semenogelase,KLK3,APS,

Full Gene Name: Prostate-specific antigen

Cellular Localisation: Secreted.

Gene ID: 354

UniProt: P07288

Pathways: Complement System