CRP ELISA Kit

Catalog No. ABIN930536

Product Details CRP ELISA Kit

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Human

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Analytical Method: Quantitative

Characteristics: ELISA kit for the detection of CRP in the research laboratory
Alternative Names: CRP ELISA kit, C-reactive protein ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Plate: Pre-coated

Assay Procedure:

The principle of this enzyme immunoassay test follows a typical twostep capture or sandwich' type assay. The assay makes use of two highly specific monoclonal antibodies: A monoclonal antibody specific for CRP is immobilized onto the microwell plate and another monoclonal antibody specific for a different region of CRP is conjugated to horse radish peroxidase (HRP). CRP from the sample and standards are allowed to bind to the plate, washed, andsubsequently incubated with the HRP conjugate. After a second washing step, the enzyme substrate is added. Theenzymatic reaction is terminated by addition of the stopping solution. The absorbance is measured on a microtiter plate reader. The intensity of the colour formed by the enzymatic reaction is directly proportional to the concentration of CRP in the sample. A set of standards is used to plot a standard curve from which the amount of CRP in patient samples and controls can be directly read.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C-Reactive Protein (CRP Products)

Background: Creactive protein (CRP) is a pentameric acute phase reactant that is synthesized by the liver. Its production is controlled primarily by interleukin 6. The serum CRP concentration may increase by up to 1000-fold with infection, trauma, surgery, and other acute inflammatory events. Chronic inflammatory disorders such as autoimmune diseases and malignancy can produce persistent high levels of serum CRP. Traditionally, CRP has been used clinically for the diagnosis and monitoring of autoimmune and infectous disorders. Recent studies have shown that chronic inflammation is an important component in the development and progression of atherosclerosis. As a result, increased serum CRP concentration are positively associated with the risk of future coronary events.
Synonyms: CRP ELISA kit, C-reactive protein ELISA kit.

Pathways: Carbohydrate Homeostasis