CRP ELISA Kit

Catalog No. ABIN930542

Product Details CRP ELISA Kit

Target: CRP (C-Reactive Protein (CRP))

Reactivity: Rat

Application: ELISA

Characteristics: ELISA kit for the detection of Rat CRP in the research laboratory
Alternative Names: Rat CRP ELISA kit, C-reactive protein ELISA kit

Application Details

Application Notes: Optimal conditions to be determined by end user

Assay Procedure:

Rat sera for testing are diluted to 1:4,000 and allowed to react with antibodies coated on specially treated microwells. After appropriate incubation, the wells are washed to remove unreacted serum proteins, and an enzymelabeled rabbit antirat CRP (conjugate) is then added to react with and tag the antigenantibody complexes. Following another incubation period, the wells are again washed to remove unreacted conjugate. A urea peroxide substrate with TMB as chromogen is added to start color development. Development of a blue color indicates a positive reaction while negative reactions appear colorless or with a trace of blue. The reaction is interrupted with a stop solution that turns the blue positive reactions to yellow. Negative reactions remain colorless or with a hint of yellow. Color intensity (absorbance) is read at a wavelength of 450nm on a spectrophotometer or ELISA reader. Assigning a value for the absorbance can be accomplished by the use of a standard curve generated by measuring twofold dilutions of the standard provided.

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: Store at 2-8 °C.

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: C-Reactive Protein (CRP Products)

Background: Creactive protein (CRP) is a pentameric acute phase reactant that is synthesized by the liver. Its production is controlled primarily by interleukin 6. The serum CRP concentration may increase by up to 1000-fold with infection, trauma, surgery, and other acute inflammatory events. Chronic inflammatory disorders such as autoimmune diseases and malignancy can produce persistent high levels of serum CRP. Traditionally, CRP has been used clinically for the diagnosis and monitoring of autoimmune and infectous disorders. Recent studies have shown that chronic inflammation is an important component in the development and progression of atherosclerosis. As a result, increased serum CRP concentration are positively associated with the risk of future coronary events.
Synonyms: Rat CRP ELISA kit, C-reactive protein ELISA kit.

Pathways: Carbohydrate Homeostasis