Histamine ELISA Kit
Quick Overview for Histamine ELISA Kit (ABIN930656)
Target
See all Histamine (HIS) ELISA KitsReactivity
Application
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Characteristics
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ELISA kit for the detection of Histamine release from heparinized whole blood in the research laboratory
Alternative Names: Histamine ELISA kit
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Histamine (HIS) ELISA Kit
HIS Reactivity: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Mouse Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Human Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Rabbit Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Chemical Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Histamine (HIS) ELISA KitHIS Reactivity: Various Species Colorimetric Sandwich ELISA 7.8-500 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Chemical Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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Application Notes
- Optimal conditions to be determined by end-user
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Assay Procedure
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Heparinized whole blood samples are incubated with different concentrations of the suspected allergen. Release of histamine will occur upon stimulation of basophilic granulocytes depending on their sensibility to the allergen. The released histamine in the supernatant is subsequently determined using a specific plasma immunoassay for the detection of histamine purchased in connection with this kit. This histamine value is related to the 100 % control (= Total Histamine) and the blank value (= Spontaneous Release). The determination of the in vitro release of histamine represents a sensitive and specific method as well as a suitable addition to routine diagnostic procedures including conventional skin testing and radioallergosorbent tests (RAST) for the determination of specific IgE anbodies in serum of atopic patients. In addition, this test also detects the releasability of the cells.
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Restrictions
- For Research Use only
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Storage
- 4 °C
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Storage Comment
- Store at 2-8 °C.
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- Histamine (HIS)
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Alternative Name
- Histamine
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Target Type
- Chemical
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Background
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Histamine is an organic nitrogen compound involved in local immune responses as well as regulating physiological function in the gut and acting as a neurotransmitter. Histamine triggers the inflammatory response. As part of an immune response to foreign pathogens, histamine is produced by basophils and by mast cells found in nearby connective tissues. Histamine increases the permeability of the capillaries to white blood cells and some proteins, to allow them to engage pathogens in the infected tissues.
Synonyms: Histamine ELISA kit.
Target See all Histamine (HIS) ELISA Kits
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