Histamine ELISA Kit
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- Target See all Histamine (HIS) ELISA Kits
- Histamine (HIS)
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Reactivity
- Human
- Detection Method
- Colorimetric
- Application
- ELISA
- Sample Type
- Plasma, Urine, Cell Culture Cells
- Sensitivity
- 0.02 ng/mL
- Characteristics
- Histamine plays a major role in the initial phase of an anaphylactic reaction. The quantification of histamine in plasma after allergen administration is of clinical interest. Histamine is part of the immune response to foreign pathogens and it increases the permeability of the capillaries to white blood cells and other proteins in order to allow them to engage foreign invaders in the affected tissues. Responsible for the biological effects of histamine in tissue are the activation of different surface receptors, for instance H1, H2 and H3. Histamine is involved in the regulating physiological function in the gut and acting as a neurotransmitter.
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Histamine (HIS) ELISA Kit
HIS Reactivity: Various Species Colorimetric Competition ELISA 1.23 ng/mL - 100 ng/mL Cell Culture Supernatant, Cell Lysate, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Human Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Mouse Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Rabbit Colorimetric Competition ELISA 1.563 ng/mL - 100 ng/mL Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Chemical Colorimetric Competition ELISA 1.56 ng/mL - 100 ng/mL Plasma, Serum
Histamine (HIS) ELISA KitHIS Reactivity: Various Species Colorimetric Sandwich ELISA 7.8-500 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
Histamine (HIS) ELISA KitHIS Reactivity: Chemical Colorimetric Competition ELISA 5.0-100 ng/mL Cell Culture Supernatant, Plasma, Serum, Tissue Homogenate
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- Application Notes
- Optimal working dilution should be determined by the investigator.
- Sample Volume
- 50 μL
- Assay Time
- 2.5 h
- Plate
- Pre-coated
- Protocol
- The competitive Histamine ELISA kit uses the microtiter plate format. Histamine is bound to the solid phase of the microtiter plate. Acylated histamine and solid phase bound histamine compete for a fixed number of antiserum binding sites. When the system is in equilibrium, free antigen and free antigen-antiserum complexes are removed by washing. The antibody bound to the solid phase histamine is detected by anti-rabbit/peroxidase. The substrate TMB / peroxidase reaction is monitored at 450 nm. The amount of antibody bound to the solid phase histamine is inversely proportional to the histamine concentration of the sample.
- Restrictions
- For Research Use only
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- Storage
- 4 °C
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- Target See all Histamine (HIS) ELISA Kits
- Histamine (HIS)
- Alternative Name
- Histamine (HIS Products)
- Target Type
- Chemical
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