Haptoglobin ELISA Kit

Catalog No. ABIN956101

Cat Haptoglobin ELISA Kit Colorimetric assay for quantification of Cat Haptoglobin.

Quick Overview for Haptoglobin ELISA Kit (ABIN956101)

Target: Haptoglobin (HP)

Reactivity: Cat

Detection Method: Colorimetric

Method Type: Sandwich ELISA

Application: ELISA

Product Details Haptoglobin ELISA Kit

Analytical Method: Quantitative

Characteristics: The Cat Haptoglobin ELISA is based on a solid phase enzyme-linked immunosorbent assay (ELISA). The assay uses affinity purified anti-cat haptoglobin antibodies for solid phase (microtiter wells) immobilization and horseradish peroxidase (HRP) conjugated anti-cat haptoglobin antibodies for detection. The test sample is diluted and incubated in the microtiter wells for 45 minutes. The microtiter wells are subsequently washed and HRP conjugate is added and incubated for 45 minutes. This results in haptoglobin molecules being sandwiched between the immobilization and detection antibodies. The wells are then washed to remove unbound HRP-labeled antibodies and TMB Reagent is added and incubated for 20 minutes at room temperature. This results in the development of a blue color. Color development is stopped by the addition of Stop Solution, changing the color to yellow, and optical density is measured spectrophotometrically at 450 nm. The concentration of haptoglobin is proportional to the optical density of the test sample.

Components: Anti-cat haptoglobin antibody coated microtiter plate with 96 wells (provided as 12 detachable strips of 8)
Enzyme Conjugate Reagent, 11 mL
Cat Haptoglobin Calibrator (lyophilized)
Wash Buffer (20X), 50 mL
Diluent (10X), 25 mL
TMB Reagent (One-Step), 11 mL
Stop Solution (1N HCl), 11 mL.

Material not included: Precision pipettes and tips
Distilled or de-ionized water
Polypropylene or glass tubes
Vortex mixer
Absorbent paper or paper towels
Micro-Plate incubator/shaker mixing speed of ~150 rpm
Plate reader with an optical density range of 0-4 at 450 nm
Graph paper (PC graphing software is optional)

Application Details

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Storage Comment: The kit should be stored at 4°C and the microtiter strips should be kept in a sealed bag with desiccant to minimize exposure to damp air. The kit will remain stable until the expiration date provided that the components are stored as described above.

Expiry Date: The expiry date is stated on the label.

Target Details for Haptoglobin

Target: Haptoglobin (HP)

Alternative Name: Haptoglobin

Background: Haptoglobin is an acute phase protein, the concentrations of which can increase two to ten fold in cat serum as a result of disease. Measurement of haptoglobin provides a convenient marker of inflammation and disease in cats.

Pathways: Transition Metal Ion Homeostasis