Urinary Diacetylspermine ELISA Kit

Catalog No. ABIN956345

This Colorimetric ELISA kit is designed for the quantitative measurement of Rodents Urinary Diacetylspermine.

Quick Overview for Urinary Diacetylspermine ELISA Kit (ABIN956345)

Target: Urinary Diacetylspermine

Reactivity: Rodents

Detection Method: Colorimetric

Method Type: Competition ELISA

Application: ELISA

Sample Type: Urine

Product Details

Purpose: The Urinary Diacetylspermine EIA is for the quantitative determination of urinary diacetylspermine in rodents.

Analytical Method: Quantitative

Characteristics: Our kit is convenient for use quantifying the amount of urinary diacetylspermine by using the ELSA method. This kit is for research use only. Highly sensitive and specific Strip type well, antigen pre-coated microplate. Assay range: 6.25-200 nM

Components: Wash Solution: 30 mL 20x concentrate
Antibody Diluent: 20 mL Antibody
Plate: 96 wells
Calibrator: 2 x 250 µL Anti-Diacetylspermine
Antibody: 60 µL 100x concentrate
HRP-Anti Rabbit IgG Antibody: 80 µL 80x concentrate
OPD (o-phenylendiamine) tablets x2
Substrate Solution: 30 mL
Stop Solution: 15 mL
Dilution Plate: 1 plate.

Material not included: Micropipette and tip
Plate washer
Plate reader

Application Details

Plate: Pre-coated

Sample Preparation:

1. Collect urine in sampling tube. Add 0.1% Na2N3 at final concentration.
   2. After centrifugation at 1500 rpm for 5 minutes, dilute the resulting supernatant over 4 times with distilled water.
   3. Measure the amount of creatinine in the remaining diluted supernatant for compensation.
   4. Prepared urine should be kept below -30°C if necessary.
   
   Procedure: Pre-reaction: Prepare the calibrator control wells containing 70 µL of anti-Diacetylspermine antibody solution and 70 µL of the 6 calibrators (200, 100, 50, 25, 12.5, 6.25 nM) in the dilution plate. Likewise prepare the experimental wells containing 70 µL of anti-Diacetylspermine antibody solution and 70 µL of prepared urinary sample in the same plate. After settlement, incubate at room temperature for 1 hour.

Calculation of Results:

In general, the concentration of urine changes easily by the amount of water taken in or by the environment. The amount of urinary creatinine depends on the amount of muscle and their measurements correlate positively.

Restrictions: For Research Use only

Handling

Storage: -20 °C

Storage Comment: All reagents should be stored at -30°C.

Target Details

Target: Urinary Diacetylspermine

Background: Polyamines are generally believed to function both in protein synthesis and DNA synthesis leading to control cell proliferation. It is reported that the total amount of urinary polyamines are elevated in cancer patients. Assays have already been developed to measure the total amount of these urinary polyamines. Recently two diacetyl-derivatives, N1, N12-diacetylspermine and N1, N8-diacetylspermine, were found to correlate to the status of disease more closely than the total amount of polyamines.