Testosterone ELISA Kit

Catalog No. ABIN996933

This Hormone Testosterone ELISA Kit is a Colorimetric ELISA Kit designed to quantify Hormone Testosterone.

Quick Overview for Testosterone ELISA Kit (ABIN996933)

Target: Testosterone

Reactivity: Hormone

Detection Method: Colorimetric

Method Type: Competition ELISA

Detection Range: 0-18 ng/mL

Application: ELISA

Sample Type: Serum

Product Details Testosterone ELISA Kit

Minimum Detection Limit: 0 ng/mL

Purpose: Testosterone Enzyme-Linked Immunosorbent Assay (ELISA) is intended for the quantitative determination of testosterone in human serum.

Analytical Method: Quantitative

Specificity: 100%

Sensitivity: 0.05 ng/mL

Application Details

Sample Volume: 10 μL

Assay Time: 1 - 2 h

Plate: Pre-coated

Restrictions: For Research Use only

Handling

Storage: 4 °C

Expiry Date: 12-14 months

Target Details for Testosterone

Target: Testosterone

Target Type: Hormone

Background: Testosterone (17 beta -hydroxyandrost-4-ene-3-one) is a C19 steroid with an unsaturated bond between C-4 and C-5, a ketone group in C-3 and a hydroxyl group in the beta position at C-17. This steroid hormone has a molecular weight of 288.4. Testosterone is the most important androgen secreted into the blood. In males, testosterone is secreted primarily by the Leydig cells of the testes, in females ca. 50 % of circulating testosterone is derived from peripheral conversion of androstenedione, ca. 25 % from the ovary and ca. 25 % from the adrenal glands. Testosterone is responsible for the development of secondary male sex characteristics and its measurements are helpful in evaluating the hypogonadal states. In women, high levels of testosterone are generally found in hirsutism and virilization, polycystic ovaries, ovarian tumors, adrenal tumors and adrenal hyperplasia. In men, high levels of testosterone are associated to the hypothalamic pituitary unit diseases, testicular tumors, congenital adrenal hyperplasia and prostate cancer.
Low levels of testosterone can be found in patients with the following diseases: Hypopituitarism, Klinefelter?s syndrome, Testicular feminization, Orchidectomy and Cryptorchidism, enzymatic defects and some autoimmune diseases.The Testosterone EIA kits are designed for the measurement of total Testosterone in human serum.