Lhca1 antibody
This Rabbit Polyclonal antibody specifically detects Lhca1 in WB. It exhibits reactivity toward Arabidopsis thaliana, Barley, Green bean, Guzmania hybrid, Moss, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinach and Zea mays and has been mentioned in 3+ publications.
(3 references)
Catalog No. ABIN5684014
Quick Overview for Lhca1 antibody (ABIN5684014)
Target
Lhca1
(psi Type I Chlorophyll A/B-Binding Protein (Lhca1))
Reactivity
Arabidopsis thaliana, Barley, Green bean, Guzmania hybrid, Moss, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinach, Zea mays
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 22 / 22 kDa for Arabidopsis thaliana
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Purification
- affinity purified
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Immunogen
- BSA-conjugated synthetic peptide derived from the Lhca1 protein of Arabidopsis thaliana UniProt: Q01667, TAIR: At3g54890. This sequence is highly conserved in Lhca1 proteins of angiosperms (monocots and dicots) and gymnosperms.
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Isotype
- IgG
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Application Notes
- Recommended Dilution: 1 : 2000 - 1 : 5000, detected with standard ECL (WB).
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Comment
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A number of degradation products may be observed when using anti-PsbA antibodies, including products having apparent molecular weights of 24kDa and16kDa. D1 degradation is a complex set of events and the products observed canbe influenced by both the extraction procedure and the physiology of the cells prior to harvest. Third, cross-linking may occur between D1 and cytochrome b559,shifting the protein higher in the gel. In cyanobacteria (PCC7942), three different bands were competed out by preincubating the antibody with the PsbA freepeptide, indicating that all bands are indeed PsbA and its precursors or breakdown product.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 µL of sterile water
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Buffer
- PBS pH 7.4
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).
: "The rice faded green leaf locus encodes protochlorophyllide oxidoreductase B and is essential for chlorophyll synthesis under high light conditions." in: The Plant journal : for cell and molecular biology, Vol. 74, Issue 1, pp. 122-33, (2013) (PubMed).
: "Stay-green protein, defective in Mendel's green cotyledon mutant, acts independent and upstream of pheophorbide a oxygenase in the chlorophyll catabolic pathway." in: Plant molecular biology, Vol. 67, Issue 3, pp. 243-56, (2008) (PubMed).
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: "Composition and structure of photosystem I in the moss Physcomitrella patens." in: Journal of experimental botany, Vol. 64, Issue 10, pp. 2689-99, (2013) (PubMed).
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- Lhca1 (psi Type I Chlorophyll A/B-Binding Protein (Lhca1))
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Background
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AGI Code: At3g54890
The light-harvesting protein Lhca1 is one of the four main and highly conserved types of chlorophyll a/b-binding proteins (Lhca1-4) of the light harvesting antenna (LHCI) of plant photosystem I. Lhca1 is imported as a precursor from the cytosol into the chloroplast. Upon insertion into the thylakoid membrane Lhca1 forms a heterodimer (LHCI-730) with Lhca4 that associates with the PSI core close to PsaG and PsaF.A biochemical characterization of the plant LHCI antenna can be found in Klimmek et al. (2005) The structure of the higher plant light harvesting complex I: in vivo characterization and structural interdependence of the Lhca proteins. Biochemistry 44: 3065-3073 -
Molecular Weight
- expected: 22 kDa, apparent: 22 kDa for Arabidopsis thaliana
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UniProt
- Q01667
Target
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