Lhcb1 antibody
This anti-Lhcb1 antibody is a Rabbit Polyclonal antibody detecting Lhcb1 in WB. Suitable for Arabidopsis thaliana. This Primary Antibody has been cited in 1 publication.
(1 reference)
Catalog No. ABIN488535
Quick Overview for Lhcb1 antibody (ABIN488535)
Target
Lhcb1 (lhcb1)
(Chlorophyll a-b binding protein (lhcb1))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Characteristics
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Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
Antibody is labelled with biotin using N-hydroxysuccinimidobiotin. Antibody potency and purity has been evaluated by immunoelectrophoresis, single radialimmunodiffusion (Ouchterlony), ELISA,immunoblotting and enzyme inhibition. -
Purification
- serum
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Immunogen
- BSA-conjugated synthetic peptide derived from Arabidopsis thaliana At1g29910 (Lhcb1.1), At1g29920 (Lhcb1.2), At1g29930 (Lhcb1.3, most expressed), At1g34430 (Lhcb1.4), and At1g34420 (Lhcb1.5)
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Isotype
- IgG
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Application Notes
- Recommended Dilution: 1 : 2500 - 1 : 5000 with standard ECL (WB).
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Comment
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This Lhcb1 antibody is directed specifically against the Arabidopsis Lhcb1 gene products, for those that would prefer higher specific activity over broader specificity offered by Agrisera older Lhcb1 antibody, AS01 004
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 100 µL of sterile water
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Buffer
- PBS pH 7.4
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Handling Advice
- Once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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Storage
- -20 °C
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: "Distinct responses of the mitochondrial respiratory chain to long- and short-term high-light environments in Arabidopsis thaliana." in: Plant, cell & environment, (2011) (PubMed).
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: "Distinct responses of the mitochondrial respiratory chain to long- and short-term high-light environments in Arabidopsis thaliana." in: Plant, cell & environment, (2011) (PubMed).
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- Lhcb1 (lhcb1) (Chlorophyll a-b binding protein (lhcb1))
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Alternative Name
- Lhcb1
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Background
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AGI Code: At1g29910, At1g29920, At1g29930, At1g34420, At1g34430
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb1 is the most abundant chlorophyll a/b-binding protein in eukaryotic phototrophs and often is coded by several nuclear genes. -
Molecular Weight
- expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana
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UniProt
- P04777, Q8VZ87, P04778, Q9C5R6, Q39141
Target
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