Lhcb2 antibody
The Rabbit Polyclonal anti-Lhcb2 antibody has been validated for IP and WB. It is suitable to detect Lhcb2 in samples from Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Green bean, Moss, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinach and Zea mays. There are 5+ publications available.
(5 references)
Catalog No. ABIN93509
Quick Overview for Lhcb2 antibody (ABIN93509)
Target
Lhcb2
(Lhcii Type II Chlorophyll A/B-Binding Protein (Lhcb2))
Reactivity
Arabidopsis thaliana, Barley, Chlamydomonas reinhardtii, Green bean, Moss, Nicotiana tabacum, Oryza sativa, Pisum sativum, Spinach, Zea mays
Host
Rabbit
Clonality
Polyclonal
Application
Immunoprecipitation (IP), Western Blotting (WB)
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Characteristics
- Expected / apparent Molecular Weight of the Antigene: 25 / 25 kDa for Arabidopsis thaliana
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Purification
- affinity purified
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Immunogen
- BSA-conjugated synthetic peptide derived from a highly conserved sequence of Lhcb2 proteins from angiosperms (monocots and dicots) and gymnosperms, including Arabidopsis thaliana Lhcb2.1 UniProt: Q9SHR7, TAIR: At2g05100, Lhcb2.2 UniProt: Q9S7J7, TAIR:At2g05070, Lhcb2.3 UniProt:Q9XF87, TAIR:At2g27690
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Isotype
- IgG
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Application Notes
- Recommended Dilution: 1 : 5000 with standard ECL (WB).
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Comment
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Immunoprecipitation has been done using Immunoprecipitation kit from Roche, Cat.No. 11 719 386 001.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 200 µL of sterile water
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Buffer
- PBS pH 7.4
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Handling Advice
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Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
Once reconstituted make aliquots to avoid repreated freeze-thaw cycles. -
Storage
- -20 °C
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: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, (2014) (PubMed).
: "LCAA, a novel factor required for magnesium protoporphyrin monomethylester cyclase accumulation and feedback control of aminolevulinic acid biosynthesis in tobacco." in: Plant physiology, Vol. 160, Issue 4, pp. 1923-39, (2012) (PubMed).
: "Light regulation to chlorophyll synthesis and plastid development of the chlorophyll-less golden-leaf privet." in: Journal of integrative plant biology, Vol. 52, Issue 9, pp. 809-16, (2010) (PubMed).
: "Core protein phosphorylation facilitates the repair of photodamaged photosystem II at high light." in: Biochimica et biophysica acta, (2008) (PubMed).
: "Phosphorylation-dependent regulation of excitation energy distribution between the two photosystems in higher plants." in: Biochimica et biophysica acta, Vol. 1777, Issue 5, pp. 425-32, (2008) (PubMed).
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: "The HIV-1 Pr55(gag) polyprotein binds to plastidial membranes and leads to severe impairment of chloroplast biogenesis and seedling lethality in transplastomic tobacco plants." in: Transgenic research, (2014) (PubMed).
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- Lhcb2 (Lhcii Type II Chlorophyll A/B-Binding Protein (Lhcb2))
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Background
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AGI Code: At2g05100
The major light-harvesting antenna complex II (LHCII) in photosynthetic eukaryotes is located in the thylakoid membrane of the chloroplast. It is a heterotrimeric complex formed by up to 3 different individual subtypes of chlorophyll a/b-binding proteins: Lhcb1, Lhcb2, and Lhcb3. Lhcb2 is often coded by several nuclear genes and is found together with Lhcb1 within the mobile LHCII trimers involved in state1-state2 transition.A molecular characterisation of the LHCII proteins can be found in Caffarri et al. (2004) A Look within LHCII: Differential Analysis of the Lhcb1-3 Complexes Building the Major Trimeric Antenna Complex of Higher-Plant Photosynthesis. Biochemistry 43 (29): 9467-9476. -
Molecular Weight
- expected: 25 kDa, apparent: 25 kDa for Arabidopsis thaliana
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UniProt
- Q9XF86, Q9XF87, Q9SHR7, Q9S7J7
Target
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