DYKDDDDK Positive Control Lysate
PC, WB
Whole Cell Lysate
Overexpression Lysate
E. coli
Reactivity: Tag
Catalog No. ABIN1607004
Supplier Product No.: w00-001-383
Quick Overview for DYKDDDDK Positive Control Lysate (ABIN1607004)
Target
DYKDDDDK Tag
Protein Species
Tag
Application
Positive Control (PC), Western Blotting (WB)
Lysed Cells
E. coli
Lysate Type
Overexpression Lysate
Lysate Fraction
Whole Cell Lysate
-
-
Species of Lysate
- Escherichia coli (E. coli)
-
Supplier Product No.
- w00-001-383
-
Supplier
- Rockland
-
Endotoxin Level
- Low Endotoxin : No
-
-
-
Application Notes
- Ready-to-use lysates are especially prepared as positive controls for separation by SDS-PAGE and subsequent western blot analysis. Lysates are prepared in denaturing buffer WITHOUT dissociating agents (i.e. no 2-mercaptoethanol or dithiothreitol has been added). If dissociating conditions are desired, add reducing agent prior to heating. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for SDS-PAGE. The product is supplied in 1X loading buffer and is ready-to-use.
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Buffer
- 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
-
Storage
- -80 °C
-
Storage Comment
- Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles.
-
Expiry Date
- Expiration date is three (3) months from date of opening.
-
-
- DYKDDDDK Tag
-
Target Type
- Tag
-
Background
- This product is intended as a positive control for use with anti-DYKDDDDK tag antibodies.
Target
-
You are here:
