Hepatitis a Virus (hav)
HAV
EIA, WB
Whole Cell Lysate
Reactivity: Hepatitis A Virus (HAV)
Catalog No. ABIN1684369
Quick Overview for Hepatitis a Virus (hav) (ABIN1684369)
Target
Hepatitis A Virus (HAV)
Protein Species
Hepatitis A Virus (HAV)
Application
Enzyme Immunoassay (EIA), Western Blotting (WB)
Lysate Fraction
Whole Cell Lysate
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Species of Lysate
- FRhK-4 Cells
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Specificity
- Hepatitis a virus (hav) antigen, >60 % viral protein
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Characteristics
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Grown in FRhK-4 Cells (Virus Strain: pHM175) .
Product manufactured in the United States, using only domestic animal materials -
Purification
- Partially Purified
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Purity
- > 60 % viral protein
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Application Notes
- Must demonstrate a major band in the region of 33 kDa and at least 1 band in the 24-27 kDa region by Western Blot and Silver Staining. The 33 kDa band must co-migrate with that in the standard lane.
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Comment
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Protein Concentration:
BCA Assay (Calculation)
Acceptable Range: > 4 µg/mL
Testing Results: 4 µg/mL
EIA Titer:
ELISA Assay:
Acceptable Range: > 270
Testing Resulst: 662
Infectivity:
Plaque Assay:
Acceptable Results: < 1 PFU/mL
Testing Results: 0 PFU/mL
Bioburden:
Acceptable Range: 0 CFU/mL
Testing Result: 0 CFU/mL -
Restrictions
- For Research Use only
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Format
- Liquid
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Buffer
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NTE Buffer: 0.01 M Tris, 0.15 M Sodium Chloride, 10 mM EDTA, pH8.0 - 8.4, 0.085% Sodium Azide.
Formalin inactivated, Formalin concentration < 1:8000 by volume -
Preservative
- Sodium azide
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Storage
- -80 °C
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Storage Comment
- Store at -80 °C. Avaoid multiple freeze/thaw cycles.
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- Hepatitis A Virus (HAV)
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Target Type
- Virus
Target
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