Jurkat nuclear extract (TPA + CI stimulated)
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- Species of Lysate
- Human Cells
- Characteristics
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Origin: Human T lymphoblast, acute T cell leukemia
- Components
- 2 x 100 μg of Jurkat nuclear extract (TPA + CI stimulated) at 2.5 μg/μl
- Lysed Cells
- Jurkat Cells
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Nuclear Extract
- Lysate Treatment
- TPA + CI stimulated
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IL25 293T Cell Transient Overexpression Lysate(Denatured)
IL25 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
FBXO11 293T Cell Transient Overexpression Lysate(Denatured)FBXO11 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PHKG2 293T Cell Transient Overexpression Lysate(Denatured)PHKG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PIK3R1 293T Cell Transient Overexpression Lysate(Denatured)PI3K p85a WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLK1 293T Cell Transient Overexpression Lysate(Denatured)PLK1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLOD2 293T Cell Transient Overexpression Lysate(Denatured)PLOD2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLSCR1 293T Cell Transient Overexpression Lysate(Denatured)PLSCR1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLCG2 293T Cell Transient Overexpression Lysate(Denatured)PLCG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLTP 293T Cell Transient Overexpression Lysate(Denatured)PLTP WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
POU4F3 293T Cell Transient Overexpression Lysate(Denatured)POU4F3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Restrictions
- For Research Use only
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- Concentration
- 2.5 μg/μL
- Buffer
- Nuclear extracts are supplied in dilution buffer (20 mM Hepes ( pH 7.9), 100 mM KCl, 1 mM MgCl2, 20 % glycerol, 0.5 mM PMSF and 0.5 mM DTT). Cells were cultured in medium supplemented with 50 ng/mL TPA (phorbol, 12-myristate, 13 acetate) and 0.5 μM calcium ionophore A23187 (CI) for two hours at 37 °C immediately prior to harvesting.
- Preservative
- Dithiothreitol (DTT)
- Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Handling Advice
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
- Storage
- -80 °C
- Storage Comment
- To ensure stability, extracts should be stored at -80°C.
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