HeLa whole-cell extract (TNF-α stimulated)
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- Species of Lysate
- Human Cells
- Characteristics
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Origin: Human Cervix Adenocarcinoma
- Components
- 2 x 125 μg of HeLa whole-cell extract (TNF-α stimulated) at 2.50 μg/μl
- Lysed Cells
- HeLa Cells
- Lysate Type
- Cell Lysate
- Lysate Fraction
- Whole Cell Lysate
- Lysate Treatment
- TNF-α stimulated
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IL25 293T Cell Transient Overexpression Lysate(Denatured)
IL25 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
FBXO11 293T Cell Transient Overexpression Lysate(Denatured)FBXO11 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PHKG2 293T Cell Transient Overexpression Lysate(Denatured)PHKG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PIK3R1 293T Cell Transient Overexpression Lysate(Denatured)PI3K p85a WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLK1 293T Cell Transient Overexpression Lysate(Denatured)PLK1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLOD2 293T Cell Transient Overexpression Lysate(Denatured)PLOD2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLSCR1 293T Cell Transient Overexpression Lysate(Denatured)PLSCR1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLCG2 293T Cell Transient Overexpression Lysate(Denatured)PLCG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLTP 293T Cell Transient Overexpression Lysate(Denatured)PLTP WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
POU4F3 293T Cell Transient Overexpression Lysate(Denatured)POU4F3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Restrictions
- For Research Use only
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- Concentration
- 2.50 μg/μL
- Buffer
- The HeLa whole-cell extracts have been collected in Lysis Buffer after a 30-minute incubation with or without TNF-α (20 ng/mL). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20 % glycerol, 1 % Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
- Handling Advice
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
- Storage
- -80 °C
- Storage Comment
- To ensure stability, extracts should be stored at -80°C.
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