HeLa whole-cell extract (TNF-α stimulated)
Quick Overview for HeLa whole-cell extract (TNF-α stimulated) (ABIN2669814)
Lysed Cells
Lysate Type
Lysate Fraction
Lysate Treatment
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Species of Lysate
- Human Cells
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Characteristics
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Origin: Human Cervix Adenocarcinoma
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Components
- 2 x 125 μg of HeLa whole-cell extract (TNF-α stimulated) at 2.50 μg/μl
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AXL 293T Cell Transient Overexpression Lysate(Denatured)
AXL WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
AVPR1A 293T Cell Transient Overexpression Lysate(Denatured)AVPR1A WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
TSPO 293T Cell Transient Overexpression Lysate(Denatured)TSPO WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
C1QBP 293T Cell Transient Overexpression Lysate(Denatured)C1QBP WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
SERPING1 293T Cell Transient Overexpression Lysate(Denatured)SERPING1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CA12 293T Cell Transient Overexpression Lysate(Denatured)CA12 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CACNG1 293T Cell Transient Overexpression Lysate(Denatured)CACNG1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CD53 293T Cell Transient Overexpression Lysate(Denatured)CD53 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CDK4 293T Cell Transient Overexpression Lysate(Denatured)CDK4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CDK4 293T Cell Transient Overexpression Lysate(Denatured)CDK4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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Restrictions
- For Research Use only
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Concentration
- 2.50 μg/μL
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Buffer
- The HeLa whole-cell extracts have been collected in Lysis Buffer after a 30-minute incubation with or without TNF-α (20 ng/mL). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 350 mM NaCl, 20 % glycerol, 1 % Igepal-CA630, 1 mM MgCl2, 0.5 mM EDTA and 0.1 mM EGTA. The protein content has been determined by a Bradford-based assay.
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Handling Advice
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Storage
- -80 °C
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Storage Comment
- To ensure stability, extracts should be stored at -80°C.
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