WI-38 nuclear extract (Forskolin stimulated)
Quick Overview for WI-38 nuclear extract (Forskolin stimulated) (ABIN2669822)
Lysed Cells
Lysate Type
Lysate Fraction
Lysate Treatment
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Species of Lysate
- Human Cells
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Characteristics
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Origin: human normal lung fibroblast
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Components
- 2 x 100 μg of WI-38 nuclear extract (Forskolin stimulated) at 2.50 μg/μl
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NLRP3 293T Cell Transient Overexpression Lysate(Denatured)
NLRP3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)TNFRSF13C WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
ZMYND19 293T Cell Transient Overexpression Lysate(Denatured)ZMYND19 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
MRGPRX2 293T Cell Transient Overexpression Lysate(Denatured)MRGPRX2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
DDIT4L 293T Cell Transient Overexpression Lysate(Denatured)DDIT4L WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
NXNL1 293T Cell Transient Overexpression Lysate(Denatured)NXNL1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
LOC124220 293T Cell Transient Overexpression Lysate(Denatured)ZG16B WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
TMEM42 293T Cell Transient Overexpression Lysate(Denatured)TMEM42 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
SIRPD 293T Cell Transient Overexpression Lysate(Denatured)SIRPD WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
RASGRP4 293T Cell Transient Overexpression Lysate(Denatured)RASGRP4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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Restrictions
- For Research Use only
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Concentration
- 2.50 μg/μL
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Buffer
- The WI-38 nuclear extracts have been collected in Lysis Buffer after a 30-minute incubation with forskolin (20 μM). The Lysis Buffer consists of 20 mM Hepes pH 7.5, 400 mM NaCl, 20 % glycerol, 0.1 mM EDTA, 10 mM NaF, 10 μM Na2MoO4, 1 mM NaVO3, 10 mM PNPP, 10 mM b-glycerophosphate, 1 mM DTT and protease inhibitors. The protein content has been determined by a Bradford-based assay.
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Preservative
- Dithiothreitol (DTT)
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Precaution of Use
- This product contains Dithiothreitol (DTT): a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- It ist recommended to aliquot the extracts into single-use fractions and then storing them at -80 °C. This eliminates repeated freeze/thaw cycles.
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Storage
- -80 °C
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Storage Comment
- To ensure stability, extracts should be stored at -80°C.
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