12 Epitope Tag Control Lysate (MBP)
Quick Overview for 12 Epitope Tag Control Lysate (MBP) (ABIN925598)
Application
Lysate Type
Lysate Fraction
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Supplier Product No.
- mb-301-0100
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Supplier
- Rockland
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Purpose
- 12 Epitope Tag Control Lysate (MBP)
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Purification
- Control lysate made in E. coli for Western blotting expresses 12 different epitope tags.
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Application Notes
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Application Note: This 12 Epitope Tag Control Lysate has been tested in SDS-PAGE and Western blot and is designed for use as a positive control for anti-epitope tag antibodies. This E. coli lysate contains a recombinant protein comprised of the following commonly used epit
Western Blot Dilution: User Optimized
Other: User Optimized
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 1.0 mg/mL
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Buffer
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Buffer: 1X SDS-PAGE Sample Buffer (62.5 mM Tris HCl, 2 % SDS, 10 % Glycerol and 0.005 % bromophenol blue, pH 6.8)
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Storage
- -80 °C
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Storage Comment
- Store vial at -70° C or COLDER. For extended storage, aliquot contents to minimize freeze/thaw cycles. Expiration date is one (1) year from date of receipt.
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Expiry Date
- 12 months
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Background
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Synonyms: Flag-tag marker, MBP-tag marker, HA-tag marker, 6XHIS-tag marker, T7-tag marker, cMyc-tag marker, V5-tag marker, S-tag marker, HSV-tag marker, VSV-tag marker, Glu-Glu-tag marker, E-tag marker, Lysate marker, Protein Marker, Loading control, epitope tag ma
Background: Epitope tags are short peptide sequences easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein's biochemical properties. Most often sequences encoding the epitope tag are included with target
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