Epitope Tag Protein Marker Lysate
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- Application
- Western Blotting (WB)
- Supplier Product No.
- mb-301-0100
- Supplier
- Rockland
- Specificity
- Liquid (in 1x Loading Buffer)
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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CD177 (human): 293T Lysate
CD177 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
CD160 (human): 293T LysateCD160 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
CD34 (human): 293T LysateCD34 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
CD81 (human): 293T LysateCD81 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
CD82 (human): 293T LysateCD82 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
CD86 (human): 293T LysateCD86 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells
IL25 293T Cell Transient Overexpression Lysate(Denatured)IL25 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PHKG2 293T Cell Transient Overexpression Lysate(Denatured)PHKG2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PIK3R1 293T Cell Transient Overexpression Lysate(Denatured)PI3K p85a WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
PLK1 293T Cell Transient Overexpression Lysate(Denatured)PLK1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- This product is designed for use as a positive control for anti-epitope tag antibodies. This E.coli lysate contains a recombinant protein comprised of the following commonly used epitope tags: MBP--T7--HSV--cMyc--VSV--Glu-Glu--V5--E-tag--Flag--S tag--HA--6XHis. A 58 kDa recombinant protein is a visualized after SDS-polyacrylamide gel electrophoresis and western blotting using an antibody recognizing any of the expressed epitope tags listed above. The E.coli lysate was prepared by lysing cells in loading buffer, heating to 95° C for 10' followed by centrifugation at 13,000 g for 15'. Thaw the lysate to room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 0.25 to 2 µL is recommended for western blotting. The product is supplied in 1X loading buffer and is ready-to-use. 62.5 mM Tris HCl, 10% Glycerol, 2% SDS, 0.05% bromphenol blue, pH 6.8 + 1% Triton 100 + 2.5 mM EDTA+5mM Tris HCl+4% (v/v) β-Mercaptoethanol.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- -20 °C/-80 °C
- Storage Comment
- Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing.
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- Background
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This product is intended as a positive control for use with anti-epitope tag antibodies. Epitope tags are short peptide sequences easily recognized by tag-specific antibodies. Due to their small size, epitope tags do not affect the tagged protein’s biochemical properties. Most often sequences encoding the epitope tag are included with target DNA at the time of cloning to produce fusion proteins. This allows anti-epitope tag antibodies to serve as universal detection reagents for any tag containing protein produced by recombinant means.
Synonyms: Flag, MBP, HA, 6XHIS, Lysate
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