CD160 (human): 293T Lysate
CD160
WB
Whole Cell Lysate
Overexpression Lysate
HEK 293T Cells
1 image
(3 references)-
- Target See all CD160 products
- CD160
- Species of Lysate
- Human Cells
- Application
- Western Blotting (WB)
- Characteristics
- CD160 is an 27 kDa glycoprotein which was initially identified with the monoclonal antibody BY55. Its expression is tightly associated with peripheral blood NK cells and CD8 T lymphocytes with cytolytic effector activity. The cDNA sequence of CD160 predicts a cysteine-rich, glycosylphosphatidylinositol- anchored protein of 181 amino acids with a single Ig- like domain weakly homologous to KIR2DL4 molecule. CD160 is expressed at the cell surface as a tightly disulfide-linked multimer. RNA blot analysis revealed CD160 mRNAs of 1.5 and 1.6 kb whose expression was highly restricted to circulating NK and T cells, spleen and small intestine. Within NK cells CD160 is expressed by CD56dimCD16+ cells whereas among circulating T cells its expression is mainly restricted to TCRgd bearing cells and to TCRab+CD8brightCD95+CD56+CD28-CD27-cells. In tissues, CD160 is expressed on all intestinal intraepithelial lymphocytes. CD160 shows a broad specificity for binding to both classical and nonclassical MHC class I molecules. Lysate of human CD160 transfected 293T cells
- Lysed Cells
- HEK 293T Cells
- Lysate Type
- Overexpression Lysate
- Lysate Fraction
- Whole Cell Lysate
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CD160 293T Cell Transient Overexpression Lysate(Denatured)
CD160 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
CD160 293T Cell Transient Overexpression Lysate(Denatured)CD160 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human
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- Application Notes
- CD160 (h): 293T Lysate is suitable as a Western Blotting positive control for human reactive CD160 antibodies. Recommended use: 10-20 µl per lane. Control 293T Lysate: 293LYS is available as a Western Blotting negative control lysate derived from non-transfected 293T cells.
- Restrictions
- For Research Use only
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- Handling Advice
- Repeated freezing and thawing should be minimized. Sample vial should be boiled once prior to use. Non-hazardous. No MSDS required.
- Storage
- -20 °C
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: "Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 490-7, (1986) (PubMed).
: "Functional expression of HLA-DP genes transfected into mouse fibroblasts." in: Nature, Vol. 313, Issue 5997, pp. 61-4, (1985) (PubMed).
: "Monoclonal antibodies to a human T-cell antigen and Ia-like antigen in the characterization of lymphoid leukemia." in: Transplantation proceedings, Vol. 13, Issue 1 Pt 2, pp. 761-6, (1981) (PubMed).
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: "Differential expression of HLA class II antigens in fetal human spleen: relationship of HLA-DP, DQ, and DR to immunoglobulin expression." in: Journal of immunology (Baltimore, Md. : 1950), Vol. 137, Issue 2, pp. 490-7, (1986) (PubMed).
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- Target
- CD160
- Alternative Name
- CD160 (CD160 Products)
- Synonyms
- CD160 Lysate, RGD1563598 Lysate, BY55 Lysate, AU045688 Lysate, By55 Lysate, NK1 Lysate, NK28 Lysate, CD160 molecule Lysate, CD160 antigen Lysate, CD160 Lysate, Cd160 Lysate
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