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A431+CAL A/OKA ACID (2HR) Lysate

Name: A431+CAL A/OKA ACID (2HR) Lysate
SKU: ABIN968887
Availability: OutOfStock

WB Whole Cell Lysate Cell Lysate A431 Cells A431 cells were grown to 80-90% confluency, serum starved overnight, then treated with 10 nM calyculin A and 500 nM okadaic acid for 2 hours at 37°C.

Catalog No. ABIN968887

Shipping to: United States

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Quick Overview for A431+CAL A/OKA ACID (2HR) Lysate (ABIN968887)

Application

Western Blotting (WB)

Lysed Cells

A431 Cells

Lysate Type

Cell Lysate

Lysate Fraction

Whole Cell Lysate

Lysate Treatment

A431 cells were grown to 80-90% confluency, serum starved overnight, then treated with 10 nM calyculin A and 500 nM okadaic acid for 2 hours at 37°C.

Species of Lysate

Human Cells

Characteristics

BD Transduction Laboratories™ A431 + Calyculin A/Okadaic Acid Cell Lysate

Purification

Purified
NLRP3 293T Cell Transient Overexpression Lysate(Denatured)
NLRP3 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

TNFRSF13C 293T Cell Transient Overexpression Lysate(Denatured)
TNFRSF13C WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

ZMYND19 293T Cell Transient Overexpression Lysate(Denatured)
ZMYND19 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

MRGPRX2 293T Cell Transient Overexpression Lysate(Denatured)
MRGPRX2 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

DDIT4L 293T Cell Transient Overexpression Lysate(Denatured)
DDIT4L WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

NXNL1 293T Cell Transient Overexpression Lysate(Denatured)
NXNL1 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

LOC124220 293T Cell Transient Overexpression Lysate(Denatured)
ZG16B WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

TMEM42 293T Cell Transient Overexpression Lysate(Denatured)
TMEM42 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

SIRPD 293T Cell Transient Overexpression Lysate(Denatured)
SIRPD WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

RASGRP4 293T Cell Transient Overexpression Lysate(Denatured)
RASGRP4 WB Whole Cell Lysate Overexpression Lysate HEK 293T Cells Reactivity: Human

Application Notes

Optimal working dilution should be determined by the investigator.

Comment

Since applications vary, each investigator should titrate the reagent to obtain optimal results.

Protocol

Cell or tissue lysates are a useful control for the western blotting application. A source that can be rich in endogenously expressed proteins, this lysate is convenient to use and provided in a denatured format allowing for immediate loading onto an SDS-PAGE gel. A431 cells were grown to 80-90 % confluency, serum starved overnight, then treated with 10 nM calyculin A and 500 nM okadaic acid for 2 hours at 37 °C.

Restrictions

For Research Use only
Format

Liquid

Concentration

1.0 mg/mL

Buffer

SDS-PAGE buffer (62mM Tris pH 6.8, 2% SDS, 0.9% b-mercaptoethanol, 0.003% bromophenol blue, 5% glycerol)

Storage

-20 °C

Storage Comment

Do not dilute or boil. Store undiluted at -20° C.