Catalog No. ABIN925595
Supplier Product No.: mb-201-0200
Quick Overview for Protein Molecular Weight Marker (ABIN925595)
Detection Method
Colorimetric
Detection Range
14-120 kDa
Application
SDS-PAGE (SDS), Western Blotting (WB)
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Minimum Detection Limit
- 14 kDa
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Supplier Product No.
- mb-201-0200
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Supplier
- Rockland
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Purpose
- This protein molecular weight marker is a cocktail containing seven purified proteins that range in size from 14 to 120 kDa and thus span a molecular weight range common to most proteins and protein subunits.
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Characteristics
- The protein mixture has been formulated to yield well-defined bands after SDS-polyacrylamide gel electrophoresis and staining with Coomassie blue or other common staining techniques. The intensity of the 67 kDa bovine albumin band has been increased to serve as a reference. The product is supplied in 1X loading buffer and is ready-to-use.
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Sterility
- Sterile filtered
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ExcelBand™ 3-color Regular Range Protein Marker
10 kDa - 180 kDa
ExcelBand™ 3-color High Range Protein Marker10 kDa - 245 kDa
ExcelBand™ Enhanced 3-color Regular Range Protein Marker10 kDa - 180 kDa
ExcelBand™ Enhanced 3-color High Range Protein Marker10 kDa - 245 kDa
ExcelBand™ 3-color Broad Range Protein Marker5 kDa - 245 kDa
ExcelBand™ 3-color Extra Range Protein Marker10 kDa - 310 kDa
AccuBand™ 100 bp DNA Ladder II100 bp - 1000 bp
AccuBand™ 100 bp DNA Marker II100 bp - 2000 bp
ExcelBand™ 1KB (0.25-10 kb) DNA Ladder250 bp - 10000 bp
ExcelBand™ 100 bp+3K DNA Ladder100 bp - 3000 bp
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Application Notes
- This product is designed for use in accurate estimation of protein molecular weight in SDS-polyacrylamide gel electrophoresis and western blotting. Thaw the cocktail at room temperature. Gently invert vial several times to ensure that the solution is homogeneous and that any precipitated material is re-dissolved. A loading volume of 5-10µl is appropriate for most gel formats. These are unstained markers optimized for use with Coomassie blue staining, but the loading volume can be adjusted for compatibility with other staining methods (i.e. silver staining). 62.5 mM Tris-HCl, pH 6.8, 2% (w/v) SDS, 250 mM NaCl, 1mM EDTA, 4% (v/v) β-Mercaptoethanol, 35% (v/v) Glycerol and 0.05% (w/v) bromophenol blue
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Comment
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in 1x Loading Buffer
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Restrictions
- For Research Use only
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Format
- Liquid
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Storage
- -20 °C
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