NRT2:1 antibody
The Rabbit Polyclonal anti-NRT2:1 antibody has been validated for WB. It is suitable to detect NRT2:1 in samples from Arabidopsis thaliana.
Catalog No. ABIN4966259
Quick Overview for NRT2:1 antibody (ABIN4966259)
Target
NRT2:1
(Nitrate Transporter 2:1 (NRT2:1))
Reactivity
Arabidopsis thaliana
Host
Rabbit
Clonality
Polyclonal
Application
Western Blotting (WB)
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Cross-Reactivity (Details)
- Not reactive in: no confirmed exceptions from predicted reactivity are currently known
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Predicted Reactivity
- Brassica juncea, Brassica napus, Brassuca rapa, Ricinus communis
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Immunogen
- KLH-conjugated synthetic peptide derived from Arabidopsis thaliana NRT2.1 sequence, UniProt: O82811, TAIR:AT1G08090
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Application Notes
- 1 : 5000 with standard ECL (WB)
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Comment
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Microsomal fraction is recommended to be used as it will have higher amount of NRT2.1 protein. If a total protein extract is used SDS should be applied from the beginning in extraction buffer as well as protease inhibitor coctail.
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Restrictions
- For Research Use only
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Format
- Lyophilized
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Reconstitution
- For reconstitution add 50 μL of sterile water.
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Storage
- -20 °C
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Storage Comment
- store lyophilized/reconstituted at -20°C, once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
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- NRT2:1 (Nitrate Transporter 2:1 (NRT2:1))
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Alternative Name
- Nitrate transporter 2.1 (NRT2.1)
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Background
- NRT2.1 is a high affinity nitrate transporter which is up-regulated by nitrate. Functions as a repressor of lateral root initiation independently of nitrate uptake. Synonymes: Protein ACH1, Protein LATERAL ROOT INITIATION 1, ACH1, ATNRT2.1, ATNRT2:1, LIN1, NITRATE TRANSPORTER 2, NITRATE TRANSPORTER 2.1, NITRATE TRANSPORTER 2:1, NRT2, NRT2.1, NRT2:1, NRT2,1AT
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Molecular Weight
- 57.7 | 45 kDa
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UniProt
- O82811
Target
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